EK1284

Human TIMP-3 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：TIMP3, SFD, TIMP-3
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：100 μL
灵敏度：1.74 pg/mL
检测范围：15.63 pg/mL - 1000 pg/mL
回收率：97% - 116%

在售SKU:70-EK1284-48, 70-EK1284-96

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文章目录[隐藏]

分子信息
TIMP3 分子靶点信息概述
TIMP3 分子靶点综述
- 人 Human TIMP3 分子靶点信息
- 人 Human TIMP3靶点分子功能（预测）

商品名	Human TIMP-3 ELISA Kit (人组织金属蛋白酶抑制剂3 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：4.3% - 4.9%；板间变异系数：4.7%-4.9%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	100 μL
灵敏度	1.74 pg/mL
检测范围	15.63 pg/mL - 1000 pg/mL
回收率	97% - 116%
平均回收率	1.04
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗TIMP-3单克隆抗体的96孔聚苯乙烯酶标板人TIMP-3冻干标准品人TIMP-3检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人TIMP-3抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的TIMP-3与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中TIMP-3的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human TIMP-3 ELISA Kit (人组织金属蛋白酶抑制剂3 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 TIMP3, SFD, TIMP-3 靶点信息

TIMP3 分子靶点信息概述

分子名：TIMP3, TIMP metallopeptidase inhibitor 3
基因家族：Tissue inhibitor of metallopeptidases
曾用名：SFD
全称：tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy, pseudoinflammatory)

TIMP3 分子靶点综述

组织金属蛋白酶抑制剂3 (TIMP-3)是人体内由TIMP3基因编码的一种蛋白质，属于金属蛋白酶基因家族的组织抑制剂。该家族有四个成员：TIMP-1、TIMP-2、TIMP-3和TIMP-4。TIMP-3是由很多类型的细胞分泌产生的分子量为30 kDa的糖蛋白。TIMP-3通过其N末端结构域与MMP活性位点形成非共价二元复合物，来抑制活性MMP介导的蛋白水解。此外，TIMP-3也是炎症因子抑制剂，负向调控TACE，促进TNF-α的释放，反过来引起机体发生炎症反应和血管重塑。该基因突变与常染色体显性遗传性Sorsby眼底营养不良有关。

人 Human TIMP3 分子靶点信息

分子名：TIMP3, TIMP metallopeptidase inhibitor 3
别称：
- HSMRK222
- K222
- K222TA2
- metalloproteinase inhibitor 3
- MIG-5 protein
- protein MIG-5
- SFD
- TIMP metallopeptidase inhibitor 3 (Sorsby fundus dystrophy, pseudoinflammatory)
- TIMP-3
- tissue inhibitor of metalloproteinase 3
- tissue inhibitor of metalloproteinases 3
基因序列：NCBI_Gene: 7078
蛋白序列：UniProtKB: P35625

人 Human TIMP3靶点分子功能（预测）

Predicted to enable metalloendopeptidase inhibitor activity and protease binding activity. Involved in negative regulation of ERK1 and ERK2 cascade; negative regulation of membrane protein ectodomain proteolysis; and positive regulation of TRAIL-activated apoptotic signaling pathway. Located in nucleus. Colocalizes with collagen-containing extracellular matrix. Implicated in Sorsby's fundus dystrophy; breast carcinoma; and urinary bladder cancer. Biomarker of several diseases, including breast cancer (multiple); preretinal fibrosis; reproductive organ cancer (multiple); thoracic aortic aneurysm; and type 2 diabetes mellitus.