EK1M03

Human MMP-3 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：MMP3, MMP-3, STMY
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：100 μL
灵敏度：0.30 pg/mL
检测范围：31.25 pg/mL - 2000 pg/mL
回收率：95% - 105%

在售SKU:70-EK1M03-48, 70-EK1M03-96

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描述

文章目录[隐藏]

分子信息
MMP3 分子靶点信息概述
MMP3 分子靶点综述
- 人 Human MMP3 分子靶点信息
- 人 Human MMP3靶点分子功能（预测）

商品名	Human MMP-3 ELISA Kit (人基质金属蛋白酶3 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：4.0% - 4.9%；板间变异系数：4.2% - 9.0%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	100 μL
灵敏度	0.30 pg/mL
检测范围	31.25 pg/mL - 2000 pg/mL
回收率	95% - 105%
平均回收率	1.01
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗MMP-3单克隆抗体的96孔聚苯乙烯酶标板人MMP-3冻干标准品 MMP-3检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人MMP-3抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的MMP-3与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中MMP-3的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长5632 nm)测定吸光度值。

Human MMP-3 ELISA Kit (人基质金属蛋白酶3 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 MMP3, MMP-3, STMY 靶点信息

MMP3 分子靶点信息概述

分子名：MMP3, matrix metallopeptidase 3
基因家族：M10 matrix metallopeptidases
曾用名：STMY1; STMY
全称：matrix metalloproteinase 3 (stromelysin 1, progelatinase); stromelysin 1

MMP3 分子靶点综述

基质金属蛋白酶3(MMP-3)，又名基质分解素-1，在人类中由MMP3基因编码。MMP3的表达主要在转录水平上调控，转录水平上的基因启动子对多种刺激物包括生长因子、细胞因子、促癌剂和原癌基因产物作出应答。MMP-3酶降解II、III、IV、IX和X型胶原蛋白、蛋白聚糖、纤维连接蛋白、层粘连蛋白和弹性蛋白。另外，MMP-3还可激活其它MMPs如MMP-1、MMP-7和MMP-9，因此MMP-3在结缔组织重塑中起关键作用。该酶参与伤口修复、动脉粥样硬化进展和肿瘤启动。MMP-3野生型和基因敲除的小鼠研究表明，MMP-3增加了外伤性损伤后的血脑屏障通透性。细胞核MMP-3可作为转录因子和蛋白酶发挥作用。

人 Human MMP3 分子靶点信息

分子名：MMP3, matrix metallopeptidase 3
别称：
- CHDS6
- matrix metallopeptidase 3 (stromelysin 1, progelatinase)
- matrix metalloproteinase 3 (stromelysin 1, progelatinase)
- matrix metalloproteinase-3
- MGC126102
- MGC126103
- MGC126104
- MMP-3
- proteoglycanase
- SL-1
- STMY
- STMY1
- STR1
- stromelysin-1
- transin-1
基因序列：NCBI_Gene: 4314
蛋白序列：UniProtKB: P08254

人 Human MMP3靶点分子功能（预测）

Enables endopeptidase activity and metallopeptidase activity. Involved in several processes, including cellular response to UV-A; negative regulation of hydrogen peroxide metabolic process; and positive regulation of protein-containing complex assembly. Predicted to be located in cytosol and mitochondrion. Implicated in several diseases, including artery disease (multiple); carcinoma (multiple); end stage renal disease; osteoarthritis; and von Hippel-Lindau disease. Biomarker of several diseases, including Takayasu's arteritis; arthritis (multiple); autoimmune disease (multiple); conjunctivochalasis; and lung disease (multiple).