EK1293

Human MSP/MST1 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

分子靶点：MST1, MSP, HGFL
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：100 μL(稀释液)
灵敏度：1.33 pg/mL
检测范围：39.06 pg/mL - 2500 pg/mL
回收率：80% - 126%

在售SKU:70-EK1293-48, 70-EK1293-96

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文章目录[隐藏]

分子信息
MST1 分子靶点信息概述
MST1 分子靶点综述
- 人 Human MST1 分子靶点信息
- 人 Human MST1靶点分子功能（预测）

商品名	Human MSP/MST1 ELISA Kit (人巨噬细胞刺激蛋白 (MSP/MST1) ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：1.6% - 3.4%；板间变异系数：2.5%-3.9%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	100 μL(稀释液)
灵敏度	1.33 pg/mL
检测范围	39.06 pg/mL - 2500 pg/mL
回收率	80% - 126%
平均回收率	1.04
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗MSP单克隆抗体的96孔聚苯乙烯酶标板人MSP冻干标准品人MSP检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人MSP抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、检测样本和生物素化的检测抗体，经过孵育，样本中存在的MSP与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP).洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中MSP的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Human MSP/MST1 ELISA Kit (人巨噬细胞刺激蛋白 (MSP/MST1) ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 MST1, MSP, HGFL 靶点信息

MST1 分子靶点信息概述

分子名：MST1, macrophage stimulating 1
别名：MSP; NF15S2
曾用名：D3F15S2; HGFL; DNF15S2
全称：hepatocyte growth factor-like

MST1 分子靶点综述

巨噬细胞刺激蛋白（MSP），也称为肝细胞生长因子样蛋白（HGFL）和MST1，是生长因子HGF家族的成员。 MSP以无活性的单链前体（pro-MSP）的形式分泌，是跨膜受体酪氨酸激酶受体（RON）的配体（RON；也称为MST1R）。自发现以来，已证明MSP在调节多种疾病模型的外周组织中的炎症中起关键作用。最近的证据还表明MSP在调节肝脂质和葡萄糖代谢中具有有益作用，从而暗示MSP作为维持代谢稳态的关键调节剂，同时抑制炎症过程。MSP诱导巨噬细胞和角质形成细胞增殖以及破骨细胞活化。它还通过巨噬细胞抑制LPS或IFN诱导的iNOS和IL-12表达，并可阻止从细胞外基质分离得到的上皮细胞发生凋亡。

人 Human MST1 分子靶点信息

分子名：MST1, macrophage stimulating 1
别称：
- D3F15S2
- DNF15S2
- hepatocyte growth factor-like protein
- hepatocyte growth factor-like protein homolog
- HGFL
- macrophage stimulating 1 (hepatocyte growth factor-like)
- macrophage stimulatory protein
- macrophage-stimulating protein
- MSP
- NF15S2
基因序列：NCBI_Gene: 4485
蛋白序列：UniProtKB: P26927

人 Human MST1靶点分子功能（预测）

Enables receptor tyrosine kinase binding activity. Involved in histone H2A-S139 phosphorylation; negative regulation of gluconeogenesis; and regulation of cAMP-dependent protein kinase activity. Located in extracellular space. Part of collagen-containing extracellular matrix.