EK1M01

Human MMP-1 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥1,600.00 – ¥2,650.00

分子靶点：MMP1, CLG
种属：人 (Human)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：20 μL
灵敏度：11.19 pg/mL
检测范围：0.16 ng/mL - 10 ng/mL
回收率：83% - 115%

在售SKU:70-EK1M01-48, 70-EK1M01-96

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库存号

描述

文章目录[隐藏]

分子信息
MMP1 分子靶点信息概述
MMP1 分子靶点综述
- 人 Human MMP1 分子靶点信息
- 人 Human MMP1靶点分子功能（预测）

商品名	Human MMP-1 ELISA Kit (人基质金属蛋白酶1 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：2.9% - 4.9%；板间变异系数：3.9% - 4.8%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	20 μL
灵敏度	11.19 pg/mL
检测范围	0.16 ng/mL - 10 ng/mL
回收率	83% - 115%
平均回收率	1.07
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗MMP-1单克隆抗体的96孔聚苯乙烯酶标板人MMP-1冻干标准品 MMP-1检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗人MMP-1抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的MMP-1与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中MMP-1的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长5632 nm)测定吸光度值。

Human MMP-1 ELISA Kit (人基质金属蛋白酶1 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述人 MMP1, CLG 靶点信息

MMP1 分子靶点信息概述

分子名：MMP1, matrix metallopeptidase 1
基因家族：Receptor ligands; M10 matrix metallopeptidases
曾用名：CLG
全称：interstitial collagenase; matrix metalloproteinase 1 (interstitial collagenase)

MMP1 分子靶点综述

基质金属蛋白酶1(MMP-1)，又名间质胶原酶和成纤维细胞胶原酶，在人类中由MMP1基因编码。MMP-1由成纤维细胞、软骨细胞、巨噬细胞、角质形成细胞、内皮细胞和成骨细胞产生。其产生受多种刺激物包括细胞因子(如EGF、白细胞介素和TNF-α)和化学物上调，受TIMP-1、TIMP-2和α2-巨球蛋白抑制。MMP-1在细胞外基质重塑中的胶原纤维降解中起重要作用，并参与多种生物过程包括类风湿性关节炎、骨关节炎、牙周病、肿瘤侵袭、血管形成、角膜溃疡、组织重塑、炎症性肠病、动脉粥样硬化和动脉瘤。另外，MMP-1同样也裂解多种底物如酪蛋白、明胶、蛋白聚糖、内功素、促肿瘤坏死因子和软骨连接蛋白。

人 Human MMP1 分子靶点信息

分子名：MMP1, matrix metallopeptidase 1
别称：
- CLG
- CLGN
- fibroblast collagenase
- interstitial collagenase
- matrix metallopeptidase 1 (interstitial collagenase)
- matrix metalloprotease 1
- matrix metalloproteinase 1
- matrix metalloproteinase 1 (interstitial collagenase)
基因序列：NCBI_Gene: 4312
蛋白序列：UniProtKB: P03956

人 Human MMP1靶点分子功能（预测）

Enables metalloendopeptidase activity. Involved in cellular response to UV-A; positive regulation of protein-containing complex assembly; and proteolysis. Predicted to be located in extracellular region. Implicated in several diseases, including artery disease (multiple); female reproductive organ cancer (multiple); intrinsic cardiomyopathy (multiple); obstructive lung disease (multiple); and urinary system cancer (multiple). Biomarker of several diseases, including artery disease (multiple); arthritis (multiple); idiopathic pulmonary fibrosis; immunoglobulin light chain amyloidosis; and systemic lupus erythematosus.