EK171EGA

EasyGo!™ Human IgG One Step ELISA Kit 检测试剂盒（酶联免疫吸附法）

¥2,190.00 – ¥3,650.00

分子靶点：IgG
种属：人 (Human)
样本类型：血清，血浆
检测样本体积：5 μL
灵敏度：0.04 ng/mL
检测范围：1.56 ng/mL - 100 ng/mL
回收率：77% - 122%

在售SKU:70-EK171EGA-48, 70-EK171EGA-96, EK171EGA

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Size
库存号

描述

商品名	Easy Go!™ Human IgG One Step ELISA Kit 检测试剂盒（酶联免疫吸附法）
检测方法	双抗夹心法
精密度	板内变异系数：8.1% - 9.0%；板间变异系数：5.8% - 7.8%
样本类型	血清，血浆
检测样本体积	5 μL
灵敏度	0.04 ng/mL
检测范围	1.56 ng/mL - 100 ng/mL
回收率	77% - 122%
平均回收率	91%
板式	96孔板，可拆
保存	试剂盒未拆开，2 - 8℃保存。已拆开，标准品-20℃保存，其它2 - 8℃保存。
运输条件	4℃蓝冰运输
组分	含微球的预包被酶标板标准品标准品稀释液显色底物终止液 20×洗液

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性捕获抗体预包被在高亲和力的酶标板上，酶标板孔中预先加入辣根过氧化物酶标记的检测抗体微球。酶标板孔中加入标准品和待测样本，经过孵育，样本中存在的待测物质与捕获抗体和检测抗体结合。洗涤去除未结合的物质后，加入显色底物（TMB），避光显色。颜色反应的深浅与样本中待测物质的浓度成正比。加入终止液终止反应，在450 nm波长 (参考波长570 - 630 nm) 测定吸光度值。