F2111703

Anti-Mouse CD117(c-Kit)，APC (Clone: ACK2) 流式抗体 (新品)

¥784.00 – ¥1,728.00

说明书

分子靶点：KIT, CD117, SCFR
种属：小鼠 (Mouse)
标记：APC
亚型：Rat IgG2b, κ
克隆号：ACK2

在售SKU, 70-AM11705-20, 70-AM11705-50, 70-AM11705-100, AM11705, 70-F2111703-25, 70-F2111703-100

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Size
库存号

描述

文章目录[隐藏]

分子信息
KIT 分子靶点信息概述
KIT 分子靶点综述
- 小鼠 Mouse Kit 分子靶点信息
- 小鼠 Mouse Kit 靶点分子功能（预测）

商品名	Anti-Mouse CD117(c-Kit)，APC (Clone: ACK2) 流式抗体
靶点	KIT, CD117, SCFR
标记	APC
仪器	适用633nm激光器的流式细胞仪
亚型	Rat IgG2b, κ
克隆号	ACK2
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 图. ICR 小鼠骨髓细胞染色 Anti-Human/MouseCD45R, FITC (AH045R01) 和 Rat IgG2b IsotypeControl, APC (CRG2b05) ( 左 ) 和 Anti-Human/MouseCD45R, FITC (AH045R01) 和 Anti-Mouse CD117(c-Kit), APC (AM11705) (右)的结果。已排除粘连细胞。

分子信息

概述小鼠 KIT, CD117, SCFR 靶点信息

KIT 分子靶点信息概述

分子名：KIT, KIT proto-oncogene, receptor tyrosine kinase
基因家族：Receptor tyrosine kinases; CD molecules; Immunoglobulin like domain containing
别名：CD117; SCFR; C-Kit
曾用名：PBT
全称：mast/stem cell growth factor receptor Kit; piebald trait; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog

KIT 分子靶点综述

CD117 也称为 c-Kit、Steel 因子受体和干细胞因子受体，这种酪氨酸激酶蛋白主要表达于造血祖细胞、肥大细胞、急性髓系白血病细胞(AML)。CD117 激活促进了这些细胞亚型的增殖和分化。缺乏或缺陷干细胞因子可导致造血祖细胞数量减少和严重贫血。CD117 也由黑色素细胞表达，并在这些细胞的信号传导和激活中起作用。

小鼠 Mouse Kit 分子靶点信息

分子名：Kit, KIT proto-oncogene receptor tyrosine kinase
别称：
- belly-spot
- Bs
- c-KIT
- CD117
- dominant spotting
- Dominant white spotting
- Fdc
- gsf spotted coat 1
- gsf spotted coat 5
- Gsfsco1
- Gsfsco5
- Gsfsow3
- phenotype like Sl or W 3
- SCO1
- SCO5
- SOW3
- spotted sterile male
- Ssm
- Steel Factor Receptor
- Tr-kit
- W
基因序列：NCBI_Gene: 16590
蛋白序列：

小鼠 Mouse Kit 靶点分子功能（预测）

Enables SH2 domain binding activity; protease binding activity; and stem cell factor receptor activity. Involved in several processes, including detection of mechanical stimulus involved in sensory perception of sound; erythropoietin-mediated signaling pathway; and hemopoiesis. Acts upstream of or within several processes, including ectopic germ cell programmed cell death; hemopoiesis; and positive regulation of macromolecule metabolic process. Located in cell-cell junction and external side of plasma membrane. Is expressed in several structures, including alimentary system; branchial arch; central nervous system; embryo mesenchyme; and genitourinary system. Used to study gastrointestinal stromal tumor; macrocytic anemia; mastocytosis; and piebaldism. Human ortholog(s) of this gene implicated in several diseases, including acute myeloid leukemia; gastrointestinal stromal tumor; germ cell cancer (multiple); piebaldism; and renal cell carcinoma. Orthologous to human KIT (KIT proto-oncogene, receptor tyrosine kinase).

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

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使用方法

全血样本：取 100 μl 抗凝血，加入 5 μl Anti-Mouse CD117 (c-Kit), APC 和其他抗体，震荡混匀，于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300 × g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号 S1001)重悬，加入 5 μl Anti-Mouse CD117 (c-Kit), APC 和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液，300 × g 离心5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。