EK2M09

Mouse Total MMP-9 ELISA Kit检测试剂盒（酶联免疫吸附法）

¥2,000.00 – ¥3,400.00

分子靶点：MMP9, MMP-9, CLG4B
种属：小鼠 (Mouse)
样本类型：血清，血浆，细胞培养上清及其他生物学样本
检测样本体积：100 μL(稀释液)
灵敏度：34.90 pg/mL
检测范围：0.16 ng/mL - 10 ng/mL
回收率：83% - 124%

在售SKU:70-EK2M09-48, 70-EK2M09-96

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库存号

描述

文章目录[隐藏]

分子信息
MMP9 分子靶点信息概述
MMP9 分子靶点综述
- 小鼠 Mouse Mmp9 分子靶点信息
- 小鼠 Mouse Mmp9 靶点分子功能（预测）

商品名	Mouse Total MMP-9 ELISA Kit (小鼠总基质金属蛋白酶9 ELISA试剂盒)
检测方法	双抗夹心法
精密度	板内变异系数：3.4% - 3.7%；板间变异系数：2.8% - 4.3%
样本类型	血清，血浆，细胞培养上清及其他生物学样本
检测样本体积	100 μL(稀释液)
灵敏度	34.90 pg/mL
检测范围	0.16 ng/mL - 10 ng/mL
回收率	83% - 124%
平均回收率	0.95
板式	96孔板，可拆
保存	试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。
运输条件	4℃蓝冰运输
组分	包被抗Mouse MMP-9单克隆抗体的96孔聚苯乙烯酶标板 Mouse MMP-9冻干标准品 Mouse MMP-9检测抗体标准品稀释液检测缓冲液(10×) 底物(TMB) 终止液洗液(20×) 封板膜

检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗小鼠MMP-9抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、检测样本和辣根过氧化物酶标记的检测抗体，经过孵育，样本中存在的MMP-9与固相抗体和检测抗体结合。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中MMP-9的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570 - 630 nm)测定吸光度值。

Mouse Total MMP-9 ELISA Kit (小鼠总基质金属蛋白酶9 ELISA试剂盒) - 标准曲线 — 标准曲线

分子信息

概述小鼠 MMP9, MMP-9, CLG4B 靶点信息

MMP9 分子靶点信息概述

分子名：MMP9, matrix metallopeptidase 9
基因家族：M10 matrix metallopeptidases
曾用名：CLG4B
全称：matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)

MMP9 分子靶点综述

基质金属蛋白酶9(MMP-9)，又名92kDaIV型胶原酶、92kDa明胶酶或者明胶酶B，是一类属于锌-金属蛋白酶家族的酶。MMP-9参与正常生理过程(如胚胎发育、繁殖、血管形成、骨骼发育、伤口愈细胞迁移、学习和记忆)和病理过程(如关节炎、脑出血和肿瘤转移)的细胞外基质降解。MMP-9和弹性蛋白酶似乎是中性粒细胞迁移通过基底膜的调节因子。MMP-9在血管和新生血管形成中起重要作用。它在人类呼吸道上皮愈合过程中被显著上调，并被发现与许多病理过程相关，如癌症、免疫和心血管疾病。在类风湿性关节炎、局灶性脑缺血案例中可发现MMP-9的水平是升高的。它的水平随着特发性房颤的发展而增加。

小鼠 Mouse Mmp9 分子靶点信息

分子名：Mmp9, matrix metallopeptidase 9
别称：
- AW743869
- B/MMP9
- Clg4b
- collagenase IVB, basement membrane, 92 kDa
- expressed sequence AW743869
- Gel B
- Gelatinase B
- gelatinase B
- MMP-9
基因序列：NCBI_Gene: 17395
蛋白序列：

小鼠 Mouse Mmp9 靶点分子功能（预测）

Enables metalloendopeptidase activity. Involved in negative regulation of epithelial cell differentiation involved in kidney development and response to amyloid-beta. Acts upstream of or within several processes, including collagen catabolic process; embryo implantation; and extracellular matrix organization. Located in collagen-containing extracellular matrix. Is expressed in several structures, including alimentary system; central nervous system; early conceptus; limb mesenchyme; and skeleton. Human ortholog(s) of this gene implicated in several diseases, including Down syndrome; anodontia; artery disease (multiple); autoimmune disease (multiple); and lung disease (multiple). Orthologous to human MMP9 (matrix metallopeptidase 9).