F41045R08

Anti-Human/Mouse CD45R, mFluor 540 (Clone: RA3-6B2) 检测试剂

¥856.00 – ¥1,960.00

说明书

分子靶点：CD45, GP180, PTPRC
种属：人 (Human); 小鼠 (Mouse)
标记：mFlour 540
亚型：Rat IgG2a, κ
克隆号：RA3-6B2

在售SKU, F41045R08, 70-F41045R08-25, 70-F41045R08-100

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Size
库存号

描述

文章目录[隐藏]

结果示例
分子信息
PTPRC 分子靶点信息概述
PTPRC 分子靶点综述

商品名	Anti-Human/Mouse CD45R, mFluor 540 (Clone: RA3-6B2) 检测试剂
靶点	CD45, GP180, PTPRC
标记	mFlour 540
仪器	适用405nm激光器的流式细胞仪
亚型	Rat IgG2a, κ
克隆号	RA3-6B2
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.2% proclin 950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

结果示例

图 - 小鼠脾脏细胞进行染色，左图为Rat IgG2a, κ Isotype control, mFluor 540的结果，右图为Anti-Human/Mouse CD45R, mFluor 540的结果。

分子信息

概述人 Human CD45, GP180, PTPRC 分子靶点信息小鼠 Mouse Itgam 分子靶点信息

PTPRC 分子靶点信息概述

分子名：PTPRC, protein tyrosine phosphatase receptor type C
基因家族：CD molecules; Fibronectin type III domain containing; Protein tyrosine phosphatases receptor type
别名：LCA; T200; GP180
曾用名：CD45

PTPRC 分子靶点综述

CD45也被称为白细胞共同抗原(leukocyte common antigen, LCA)，由一类结构相似，分子量较大(180 kDa、190 kDa、205 kDa和200 kDa)的跨膜蛋白组成，广泛存在于所有白细胞表面(包括淋巴细胞、粒细胞和单核细胞)，但不表达于成熟的红细胞、血小板、骨髓中的成熟红系细胞以及非造血组织。CD45胞浆区段具有蛋白质酪氨酸磷酸酶的作用，能使底物P56lck和P59fyn上酪氨酸脱磷酸而激活，在细胞的信息传导中发挥重要作用，CD45是细胞膜上信号传导的关键分子，在淋巴细胞的发育成熟，功能调节及信号传递中具有重要意义。CD45的分布可作为某些T细胞亚群的分类标志。

人 Human PTPRC 分子靶点信息

分子名：PTPRC, protein tyrosine phosphatase receptor type C
别称：
- B220
- CD45
- CD45 antigen
- CD45R
- glycoprotein
- GP180
- L-CA
- LCA
- leukocyte common antigen
- leukocyte-common antigen
- LY5
- protein tyrosine phosphatase, receptor type, c polypeptide
- receptor-type tyrosine-protein phosphatase C
- T200
- T200 glycoprotein
- T200 leukocyte common antigen
基因序列：NCBI_Gene: 5788
蛋白序列：UniProtKB: P08575

人 Human PTPRC靶点分子功能（预测）

Enables cytoskeletal protein binding activity; protein kinase binding activity; and protein tyrosine phosphatase activity. Involved in several processes, including hematopoietic or lymphoid organ development; positive regulation of cell population proliferation; and regulation of protein kinase activity. Located in bleb and external side of plasma membrane. Implicated in hepatitis C; multiple sclerosis; severe combined immunodeficiency; and severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-positive, Nk cell-positive.

小鼠 Mouse Ptprc 分子靶点信息

分子名：Ptprc, protein tyrosine phosphatase, receptor type, C
别称：
- B220
- CD45
- Cd45
- CD45 antigen
- Ly-5
- lymphocyte antigen 5
- Lyt-4
- T-lymphocyte antigen 4
- T200
基因序列：NCBI_Gene: 19264
蛋白序列：

小鼠 Mouse Ptprc 靶点分子功能（预测）

Enables several functions, including heparan sulfate proteoglycan binding activity; heparin binding activity; and protein tyrosine phosphatase activity. Involved in several processes, including lymphocyte differentiation; positive regulation of macromolecule metabolic process; and regulation of signal transduction. Acts upstream of or within several processes, including lymphocyte differentiation; positive regulation of lymphocyte activation; and regulation of protein phosphorylation. Located in external side of plasma membrane; focal adhesion; and membrane raft. Is expressed in several structures, including 3rd branchial arch; alimentary system; cardiovascular system; hemolymphoid system; and placenta. Used to study systemic lupus erythematosus. Human ortholog(s) of this gene implicated in hepatitis C; multiple sclerosis; severe combined immunodeficiency; and severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-positive, Nk cell-positive. Orthologous to human PTPRC (protein tyrosine phosphatase receptor type C).

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

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使用方法

全血样本：取 100 μl 抗凝血，加入 5 μl Anti-Human/Mouse CD45R, mFluor 540 和其他抗体，震荡混匀，于室温避光孵育15 分钟或2 - 8℃避光孵育30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300×g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号S1001)重悬，加入5 μl Anti-Human/Mouse CD45R, mFluor 540和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液，300 × g 离心5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。