F1105604

Anti-Human CD56, PerCP-Cy5.5 (Clone: B-A19) 流式抗体 (新品)

¥1,296.00 – ¥2,880.00

说明书

分子靶点：CD56, NCAM1, NCAM
种属：人 (Human)
标记：PerCP-Cy5.5
亚型：Mouse IgG1, κ
克隆号：B-A19

在售SKU:70-F1105604-25, 70-F1105604-100

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Size
库存号

描述

文章目录[隐藏]

分子信息
NCAM1 分子靶点信息概述
NCAM1 分子靶点综述
- 人 Human NCAM1 分子靶点信息
- 人 Human NCAM1靶点分子功能（预测）

商品名	Anti-Human CD56, PerCP-Cy5.5 (Clone: B-A19) 流式抗体
靶点	CD56, NCAM1, NCAM
标记	PerCP-Cy5.5
仪器	适用488nm激光器的流式细胞仪
亚型	Mouse IgG1, κ
克隆号	B-A19
推荐应用	流式检测
用量	5 μL /Test
纯化	亲和层析
储存液	含 0.2% BSA 和 0.02% ProClin950 的磷酸盐缓冲液(pH7.2)
保存条件	2 - 8℃避光，切勿冻存

分子信息

概述人 CD56, NCAM1, NCAM 靶点信息

NCAM1 分子靶点信息概述

分子名：NCAM1, neural cell adhesion molecule 1
基因家族：CD molecules; Fibronectin type III domain containing; I-set domain containing; Ig-like cell adhesion molecule family
别名：NCAM; CD56

NCAM1 分子靶点综述

CD56也称为神经细胞黏着分子(Neural Cell Adhesion molecule, NCAM)、Leu-19或NKH1，是分子量为140 kD的跨膜糖蛋白。CD56表达于神经元，在神经细胞的同质粘附中发挥作用。在造血系统中，它表达于NK和NKT细胞，在细胞粘附中发挥作用。

人 Human NCAM1 分子靶点信息

分子名：NCAM1, neural cell adhesion molecule 1
别称：
- antigen recognized by monoclonal antibody 5.1H11
- CD56
- MSK39
- N-CAM-1
- NCAM
- NCAM-1
- neural cell adhesion molecule, NCAM
基因序列：NCBI_Gene: 4684
蛋白序列：UniProtKB: P13591

人 Human NCAM1靶点分子功能（预测）

Predicted to enable virus receptor activity. Predicted to be involved in commissural neuron axon guidance; modulation of chemical synaptic transmission; and regulation of semaphorin-plexin signaling pathway. Predicted to act upstream of or within several processes, including homotypic cell-cell adhesion; positive regulation of calcium-mediated signaling; and regulation of exocyst assembly. Located in cytosol and external side of plasma membrane. Implicated in bipolar disorder; middle cerebral artery infarction; and pancreatic cancer. Biomarker of several diseases, including acute promyelocytic leukemia; brain glioblastoma multiforme; cholangiocarcinoma; insulinoma; and ovarian serous carcinoma.

操作步骤

文章目录[隐藏]

使用方法
注意事项
FCM流式检测常见问题
查看更多FCM流式检测相关问题

下载本产品说明书

使用方法

全血样本：取 100 μl 抗凝血，加入 5 μl Anti-Human CD56, PerCP-Cy5.5 和其他抗体，震荡混匀，于室温避光孵育 15 分钟或 2 - 8℃避光孵育 30 分钟。根据红细胞裂解液说明书进行操作，去除红细胞。300 × g 离心 5 - 10 分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
其他样本：将 1×10⁵ - 10⁸ 个单细胞经适当离心后，取适量流式染色缓冲液 (目录号 S1001)重悬，加入 5 μl Anti-Human CD56, PerCP-Cy5.5 和其他抗体，使反应终体积为 100 μl。震荡混匀，于室温避光孵育15 分钟或 2 - 8℃避光孵育 30 分钟。每管加入 1 - 2 ml 流式染色缓冲液，300 × g 离心 5 - 10分钟，弃上清，加入 500 μl 流式染色缓冲液重悬，上机检测。
实验中，可对抗体进行优化滴定以获得最佳效果。