Novus细胞自噬常用产品汇总

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http://www.liankebio.com/article-information_Tech-727.html

作者:联科生物 发布日期:2016-09-08 09:00


1. 什么是自噬?

自噬(英文名autophagy)一词合成于希腊语词根:“auto”(意为“自身”)和“phagy”(意为“吃”),意即细胞的自我消化。自噬是凋亡之外的第二种程序性细胞死亡方式,是进化过程中保留下来的高度保守的自我保护机制,参与自噬的基因从单细胞的酵母到植物、脊椎动物和人中均较为保守。

2. 自噬研究热度分析

2004年12月出版的《Science》杂志将自噬的研究列为2005年科技领域的六大热点之首。2005年,《Autophagy》杂志创刊,标志着自噬的研究已经得到了国际的公认和重视。在PubMed数据库中收录的自噬相关的文献,从1990年至2016年大约为25000余篇,而最近5年,相关的文献数量达到了17000余篇,呈现了爆炸式增长,可以预计与凋亡一样,自噬的研究将会继续呈现上升趋势(图1)。

图1:1990年至今各年收录于PubMed中的自噬(Autophagy)相关报道的文献数量

3. 自噬研究常用产品

3.1 LC3

厂商 目录号 产品名称 抗体来源 应用 交叉反应性 引用文献
Novus H6-NB100-2220 LC3 Antibody Rabbit WB, ICC/IF, IHC,more Hu,Ms,Rt,more 450
Novus H6-NB100-2331 LC3 Antibody Rabbit IHC-P,IP, WB,more Hu,Ms,Rt,more 151
Novus H6-NB600-1384 LC3B Antibody Rabbit WB, FCM, IHC,more Hu,Ms,Rt,more 147
Novus H6-NBP1-19167 LC3 Antibody Rabbit WB, FCM, IHC,more Hu,Ms,Rt,more 14

Hu: human; Ms: mouse; Rt: rat;WB: Western Blot ;FCM: flowcytometry; IHC: immunohistochemistry;

Fig1: Western Blot: LC3B/MAP1LC3B Antibody [NB100-2220] - analysis of LC3 in Huh-7 and SMMC-7721 cells using anti-LC3B antibody. Image from verified customer review. Fig2: Immunohistochemistry-Paraffin: LC3/MAP1LC3A Antibody [NB100-2331] - Analysis in mouse renal tissue. Image courtesy of an anonymous customer review.

Novus在细胞自噬方面享有极高的声誉,其产品被大量顶级期刊如Cell,Nature引用,部分文献如下:

1. [LC3, NB100-2220] Wlodarska M, Thaiss CA, Nowarski R et al. NLRP6 Inflammasome Orchestrates the Coloni Host-Microbial Interface by Regulating Goblet Cell Mucus Secretion. Cell. 2014, PMID: 24581500. (WB, Mouse)

2. [LC3, NB100-2220] Chin RM, Fu X, Pai MY et al. The metabolite alpha-ketoglutarate extends lifespan by inhibiting ATP synthase and TOR. Nature. 2014, PMID: 24828042. (WB, Human)

3. [LC3, NB100-2220] Zavodszky E, Seaman MN, Moreau K et al. Mutation in VPS35 associated with Parkinson’s disease impairs WASH complex association and inhibits autophagy. Nature Commun. 2014, PMID: 24819384. (WB,Human)

4. [LC3, NB100-2220] Fang EF, Scheibye-Knudsen M, Brace LE et al. Defective Mitophagy in XPA via PARP-1 Hyperactivation and NAD(+)/SIRT1 Reduction. Cell. 2014, PMID: 24813611. (WB, ICC/Human, Mouse, Rat)

5. [LC3, NB100-2220] Bingol B, Tea JS, Phu L et al. The mitochondrial deubiquitinase USP30 opposes parkin-mediated mitophagy. Nature. 2014, PMID: 24896179. (WB, ICC/Human)

6. [LC3, NB100-2220] Shanware NP, Bray K, Eng CH et al. Glutamine deprivation stimulates mTOR-JNK-dependent chemokine secretion. Nature Commun. 2014, PMID: 25254627.

7. [LC3B, NB600-1384] Meunier E, Dick MS, Dreier RF et al. Caspase-11 activation requires lysis of pathogen-containing vacuoles by IFN-induced GTPases. Nature 2014, PMID: 24739961. (ICC/Mouse)

8. [LC3B, NB600-1384] Lee JM, Wagner M, Xiao R et al. Nutrient-sensing nuclear receptors coordinate autophagy. Nature. 2014, PMID: 25383539.

9. [LC3, NB100-2331] Zhang L, Chen X, Sharma P et al. HACE1-dependent protein degradation provides cardiac protection in response to haemodynamic stress. Nature Commun. 2014. PMID: 24614889. (WB, Mouse)
3.2 ATG

厂商 目录号 产品名称 抗体来源 应用 交叉反应性 引用文献
Novus H6-NB110-53818 ATG5 Antibody Rabbit WB, ICC/IF, IHC,more Hu,Ms,Rt,more 116
Novus H6-NB110-56893 ATG9A Antibody Rabbit WB, ICC/IF, IHC,more Hu,Ms,Rt,more 10
Novus H6-NBP2-24682 ATG7 Antibody Rabbit WB, IHC-P Hu 2
Novus H6-NB600-1274 ATG7 Antibody Rabbit WB, ELISA, ICC/IF Hu 3
Fig3: Immunohistochemistry-Paraffin: ATG5 Antibody [NB110-53818] - Staining of mouse knee. Image from verified customer review

ATG部分引用文献:
1.[ATG5, NB110-53818] Bejarano E, Yuste A, Patel B et al. Connexins modulate autophagosome biogenesis.Nature.Cell Biol. 2014, PMID: 24705551. (WB, IP, Mouse)

2. [ATG5, NB110-53818] Fang EF, Scheibye-Knudsen M, Brace LE et al. Defective Mitophagy in XPA via PARP-1 Hyper activation and NAD(+)/SIRT1 Reduction. Cell. 2014, PMID: 24813611. (WB, Mouse).
3.3 Bcl-2

厂商 目录号 产品名称 抗体来源 应用 交叉反应性 引用文献
Novus H6-NBP2-15200 Bcl-2 Antibody Mouse WB, IHC, IP,more Hu,Rt 6
Novus H6-NB100-56098 Bcl-2 Antibody Rabbit WB, IHC, IHC-P, IP Hu,Ms,more 5
Novus H6-NB100-56101 Bcl-2 Antibody Rabbit WB, IHC, IHC-P, IP Hu,Ms,Rt,more 2

3.4 Beclin 1

厂商 目录号 产品名称 抗体来源 应用 交叉反应性 引用文献
Novus H6-NB500-249 Beclin 1 Antibody Rabbit WB, IHC, IHC-P, IP Hu,Ms,Rt 60
Novus H6-NB110-87318 Beclin 1 Antibody Rabbit WB,IHC,ICC/IF Hu,Ms,More 20
Novus H6-NBP1-00085 Beclin 1 Antibody Mouse WB, IHC, IHC-P, IP Hu,Ms,More 16
Fig4: Western Blot: Beclin 1/ATG6 Antibody [NB500-249] - WB analysis of Belclin1. Lane 1 human brain and Lane 2 mouse brain. Fig5:Immunocytochemistry/Immunofluorescence: Beclin 1/ATG6 Antibody [NB500-249] - Beclin 1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Beclin 1文献引用:
1.[Beclin 1, NB500-249] Pampliega O, Orhon I, Patel B et al. Functional interaction between autophagy andciliogenesis. Nature. 2013, PMID: 24089209. (ICC/Mouse)
3.5 p70 S6K

厂商 目录号 产品名称 规格
eBioscience 85-86051-11 p70 S6K (Total) InstantOne™ ELISA 96 tests
eBioscience 85-86052-11 phospho-p70 S6K (Thr389) InstantOne™ ELISA 96 tests
eBioscience 85-86053-11 p70 S6K (Total/Phospho) InstantOne™ ELISA 96 tests

4. 自噬诱导剂和抑制剂

厂商 目录号 产品名称 规格 作用
MultiSciences A001 Rapamycin 1 mg 自噬诱导剂,对mTOR的抑制模拟细胞饥饿,阻断细胞生长和增殖需要的信号传导。
MultiSciences A002-25 3-Methyladenine 25 mg 广泛应用的细胞自噬抑制剂,可以抑制PI3K活性,抑制自噬过程的初始阶段。
A002-50 3-Methyladenine 50 mg
MultiSciences A003 LY294002 1 mg 特异性抑制P13K信号途径,包括常见的抑制Akt磷酸化等,阻断自噬体形成。
MultiSciences A004 Bafilomycin A1 10ul 膜ATPase抑制剂家族,阻止自噬体和溶酶体的膜融合。

产品特点:进口分装,高纯度(>99%),现货供应,价格便宜,品质保证。

更多自噬产品请点击进入:联科生物自噬产品库???????联科生物代理截止时间:2017年6月30日